Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-N-tosyl-L-lysine chloromethane | inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus | |
diisopropylphosphofluoridate | irreversible inhibition | Bos taurus | |
inhibitor-chymotrypsin complex | no inhibition of trypsin-like and chymotrypsin-like activity by EDTA, Ca2+, and benzamidine | Bos taurus | |
Kunitz type soybean trypsin inhibitor | inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus | |
phenylmethanesulfonyl fluoride | irreversible inhibition | Bos taurus | |
Trypsin inhibitor | from bovine lungs, from Lima beans, inhibitory effect on chymotrypsin-like and trypsin-like activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
FMRF-NH2 | pH 8.0, 37°C <2> | Bos taurus | |
0.28 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
0.8 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
1 | - |
LEEELKPLEEVLNL | pH 8.0, 37°C | Bos taurus | |
1.1 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.2 | - |
alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
10.4 | - |
alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Bos taurus | enzyme belongs to the serine protease subfamily | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P80219 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro | active site serine | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | - |
Bos taurus | - |
mucosa | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
purified enzyme, substrate Ac-Leu-Lys-Arg-4-nitroanilide | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-acetyl-Leu-Leu-Arg + 4-nitroaniline | - |
? | |
alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-acetyl-Leu-Lys + Arg + 4-nitroaniline | - |
? | |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg + 4-nitroaniline | - |
? | |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg + 4-nitroaniline | - |
? | |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ala-Ala-Phe + 4-nitroaniline | - |
? | |
bovine serum albumin + H2O | enzyme cleaves the bonds Phe26-Ser27, Lys40-Leu41, Lys63-Ser64, Lys209-Ala210, Lys230-Leu231, Lys237-Val238, and Tyr409-Thr410 | Bos taurus | fragments of bovine serum albumin | - |
? | |
FMRF-NH2 + H2O | - |
Bos taurus | ? | - |
? | |
glucagon + H2O | enzyme cleaves the bonds Lys12-Arg13 and Arg18-Ala19 | Bos taurus | fragments of glucagon | - |
? | |
LEEELKPLEEVLNL + H2O | - |
Bos taurus | LEEELKPL + EEVLNL | - |
? | |
polypeptide + H2O | - |
Bos taurus | peptides | - |
? | |
polypeptide + H2O | enzyme belongs to the serine protease subfamily | Bos taurus | peptides | - |
? | |
porcine proinsulin + H2O | enzyme cleaves the bonds Lys-29-Thr30, Arg31-Arg32, Arg32-Glu33, Leu56-Ala57, Lys64-Arg65, and Arg66-Gly67 | Bos taurus | fragments of porcine proinsulin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
duodenase I | - |
Bos taurus |
duodenase serine protease | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
alpha-N-acetyl-Leu-Leu-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
1.2 | - |
alpha-N-acetyl-Leu-Lys-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.6 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
4.2 | - |
FMRF-NH2 | pH 8.0, 37°C | Bos taurus | |
14.4 | - |
alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
60 | - |
LEEELKPLEEVLNL | pH 8.0, 37°C | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.2 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Kunitz soybean trypsin inhibitor | pH 8.0, 37°C | Bos taurus |